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1
Fusion Protein Technologies for Biopharmaceuticals Applications and Challenges
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Fusion Protein Technologies for Biopharmaceuticals Applications and Challenges

Stefan R. Schmidt

2013

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2
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Article
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Local tertiary structure probing of ribonucleoprotein particles by nuclease fusion proteins

Ohmayer, Uli ; Perez-Fernandez, Jorge ; Hierlmeier, Thomas ; Pöll, Gisela ; Williams, Lydia ; Griesenbeck, Joachim ; Tschochner, Herbert ; Milkereit, Philipp

PloS one, 2012, Vol.7(8), pp.e42449 [Peer Reviewed Journal]

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3
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Construction of chimeric dual-chain avidin by tandem fusion of the related avidins

Riihimäki, Tiina A ; Kukkurainen, Sampo ; Varjonen, Suvi ; Hörhä, Jarno ; Nyholm, Thomas K M ; Kulomaa, Markku S ; Hytönen, Vesa P

PloS one, 2011, Vol.6(5), pp.e20535 [Peer Reviewed Journal]

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4
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Reconfiguration of the proteasome during chaperone-mediated assembly

Park, Soyeon ; Li, Xueming ; Kim, Ho ; Singh, Chingakham ; Tian, Geng ; Hoyt, Martin ; Lovell, Scott ; Battaile, Kevin ; Zolkiewski, Michal ; Coffino, Philip ; Roelofs, Jeroen ; Cheng, Yifan ; Finley, Daniel

Nature, May 23, 2013, Vol.497(7450), pp.512-6 [Peer Reviewed Journal]

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5
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Construction of a xylanase A variant capable of polymerization

Szabó, Veronika ; Muskotál, Adél ; Tóth, Balázs ; Mihovilovic, Marko D ; Vonderviszt, Ferenc

PloS one, 2011, Vol.6(9), pp.e25388 [Peer Reviewed Journal]

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6
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Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems

Reuten, Raphael ; Nikodemus, Denise ; Oliveira, Maria B ; Patel, Trushar R ; Brachvogel, Bent ; Breloy, Isabelle ; Stetefeld, Jörg ; Koch, Manuel

PloS one, 2016, Vol.11(3), pp.e0152386 [Peer Reviewed Journal]

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7
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One-step purification of recombinant human amelogenin and use of amelogenin as a fusion partner

Svensson Bonde, Johan ; Bulow, Leif

PloS one, 2012, Vol.7(3), pp.e33269 [Peer Reviewed Journal]

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8
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Live imaging of mitosomes and hydrogenosomes by HaloTag technology

Martincová, Eva ; Voleman, Luboš ; Najdrová, Vladimíra ; De Napoli, Maximiliano ; Eshar, Shiri ; Gualdron, Melisa ; Hopp, Christine S ; Sanin, David E ; Tembo, Dumizulu L ; Van Tyne, Daria ; Walker, Dawn ; Marcinčiková, Michaela ; Tachezy, Jan ; Doležal

PloS one, 2012, Vol.7(4), pp.e36314 [Peer Reviewed Journal]

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9
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Atomic resolution structure of a protein prepared by non-enzymatic His-tag removal. Crystallographic and NMR study of GmSPI-2 inhibitor

Kopera, Edyta ; Bal, Wojciech ; Lenarčič Živkovič, Martina ; Dvornyk, Angela ; Kludkiewicz, Barbara ; Grzelak, Krystyna ; Zhukov, Igor ; Zagórski-Ostoja, Włodzimierz ; Jaskolski, Mariusz ; Krzywda, Szymon

PloS one, 2014, Vol.9(9), pp.e106936 [Peer Reviewed Journal]

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10
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A novel chimeric avidin with increased thermal stability using DNA shuffling

Taskinen, Barbara ; Airenne, Tomi T ; Jänis, Janne ; Rahikainen, Rolle ; Johnson, Mark S ; Kulomaa, Markku S ; Hytönen, Vesa P

PloS one, 2014, Vol.9(3), pp.e92058 [Peer Reviewed Journal]

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11
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An albumin-derived peptide scaffold capable of binding and catalysis

Luisi, Immacolata ; Pavan, Silvia ; Fontanive, Giampaolo ; Tossi, Alessandro ; Benedetti, Fabio ; Savoini, Adriano ; Maurizio, Elisa ; Sgarra, Riccardo ; Sblattero, Daniele ; Berti, Federico

PloS one, 2013, Vol.8(2), pp.e56469 [Peer Reviewed Journal]

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12
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Recombinant Passenger Proteins Can Be Conveniently Purified by One-Step Affinity Chromatography

Wang, Hua-Zhen ; Chu, Zhi-Zhan ; Chen, Chang-Chao ; Cao, Ao-Cheng ; Tong, Xin ; Ouyang, Can-Bin ; Yuan, Qi-Hang ; Wang, Mi-Nan ; Wu, Zhong-Kun ; Wang, Hai-Hong ; Wang, Sheng-Bin

PloS one, 2015, Vol.10(12), pp.e0143598 [Peer Reviewed Journal]

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13
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Modified recombinant proteins can be exported via the Sec pathway in Escherichia coli

Chen, Nan ; Hong, Fu-Lin ; Wang, Hai-Hong ; Yuan, Qi-Hang ; Ma, Wan-Yan ; Gao, Xu-Na ; Shi, Rui ; Zhang, Rui-Juan ; Sun, Chang-Sheng ; Wang, Sheng-Bin

PloS one, 2012, Vol.7(8), pp.e42519 [Peer Reviewed Journal]

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14
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Pharmacokinetics and pharmacodynamics of recombinant human EPO-Fc fusion protein in vivo

Shi, Xunlong ; Yang, Jianjun ; Zhu, Haiyan ; Ye, Li ; Feng, Meiqing ; Li, Jiyang ; Huang, Hai ; Tao, Qun ; Ye, Dan ; Sun, Lee-Hwei K ; Sun, Bill N C ; Sun, Cecily R Y ; Han, Guizhen ; Liu, Yuanyuan ; Yao, Minghui ; Zhou, Pei ; Ju, Dianwen

PloS one, 2013, Vol.8(8), pp.e72673 [Peer Reviewed Journal]

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15
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Activity Augmentation of Amphioxus Peptidoglycan Recognition Protein BbtPGRP3 via Fusion with a Chitin Binding Domain

Wang, Wen-Jie ; Cheng, Wang ; Luo, Ming ; Yan, Qingyu ; Yu, Hong-Mei ; Li, Qiong ; Cao, Dong-Dong ; Huang, Shengfeng ; Xu, Anlong ; Mariuzza, Roy A ; Chen, Yuxing ; Zhou, Cong-Zhao

PloS one, 2015, Vol.10(10), pp.e0140953 [Peer Reviewed Journal]

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16
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A phosphorylation tag for uranyl mediated protein purification and photo assisted tag removal

Zhang, Qiang ; Jørgensen, Thomas J D ; Nielsen, Peter E ; Møllegaard, Niels Erik

PloS one, 2014, Vol.9(3), pp.e91138 [Peer Reviewed Journal]

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17
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Cellular effects of HER3-specific affibody molecules

Göstring, Lovisa ; Malm, Magdalena ; Höidén-Guthenberg, Ingmarie ; Frejd, Fredrik Y ; Ståhl, Stefan ; Löfblom, John ; Gedda, Lars

PloS one, 2012, Vol.7(6), pp.e40023 [Peer Reviewed Journal]

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18
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Construction of improved tools for protein localization studies in Streptococcus pneumoniae

Henriques, Mafalda X ; Catalão, Maria João ; Figueiredo, Joana ; Gomes, João Paulo ; Filipe, Sergio R

PloS one, 2013, Vol.8(1), pp.e55049 [Peer Reviewed Journal]

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19
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Milligram production and biological activity characterization of the human chemokine receptor CCR3

Wang, Mingqing ; Ge, Baosheng ; Li, Renmin ; Wang, Xiaoqiang ; Lao, Jun ; Huang, Fang

PloS one, 2013, Vol.8(6), pp.e65500 [Peer Reviewed Journal]

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20
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Potential pitfalls and solutions for use of fluorescent fusion proteins to study the lysosome

Huang, Ling ; Pike, Douglas ; Sleat, David E ; Nanda, Vikas ; Lobel, Peter

PloS one, 2014, Vol.9(2), pp.e88893 [Peer Reviewed Journal]

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21
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Significance of relative position of cellulases in designer cellulosomes for optimized cellulolysis

Stern, Johanna ; Kahn, Amaranta ; Vazana, Yael ; Shamshoum, Melina ; Moraïs, Sarah ; Lamed, Raphael ; Bayer, Edward A

PloS one, 2015, Vol.10(5), pp.e0127326 [Peer Reviewed Journal]

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22
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Biotechnological Fluorescent Ligands of the Bradykinin B1 Receptor: Protein Ligands for a Peptide Receptor

Charest-Morin, Xavier ; Marceau, François

PloS one, 2016, Vol.11(2), pp.e0148246 [Peer Reviewed Journal]

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23
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Comparative study of somatostatin-human serum albumin fusion proteins and natural somatostatin on receptor binding, internalization and activation

Peng, Ying ; Deng, Lili ; Ding, Yuedi ; Chen, Quancheng ; Wu, Yu ; Yang, Meilin ; Wang, Yaping ; Fu, Qiang

PloS one, 2014, Vol.9(2), pp.e89932 [Peer Reviewed Journal]

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24
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Stabilization by fusion to the C-terminus of hyperthermophile Sulfolobus tokodaii RNase HI: a possibility of protein stabilization tag

Takano, Kazufumi ; Okamoto, Tomohiro ; Okada, Jun ; Tanaka, Shun-Ichi ; Angkawidjaja, Clement ; Koga, Yuichi ; Kanaya, Shigenori

PloS one, 19 January 2011, Vol.6(1), pp.e16226 [Peer Reviewed Journal]

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25
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Mouse-hamster chimeric prion protein (PrP) devoid of N-terminal residues 23-88 restores susceptibility to 22L prions, but not to RML prions in PrP-knockout mice

Uchiyama, Keiji ; Miyata, Hironori ; Yano, Masashi ; Yamaguchi, Yoshitaka ; Imamura, Morikazu ; Muramatsu, Naomi ; Das, Nandita Rani ; Chida, Junji ; Hara, Hideyuki ; Sakaguchi, Suehiro

PloS one, 2014, Vol.9(10), pp.e109737 [Peer Reviewed Journal]

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26
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Exploiting ligand-protein conjugates to monitor ligand-receptor interactions

Haruki, Hirohito ; Gonzalez, Monica Rengifo ; Johnsson, Kai

PloS one, 2012, Vol.7(5), pp.e37598 [Peer Reviewed Journal]

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27
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Radiotherapy combined with the immunocytokine L19-IL2 provides long-lasting antitumor effects

Zegers, Catharina M L ; Rekers, Nicolle H ; Quaden, Dana H F ; Lieuwes, Natasja G ; Yaromina, Ala ; Germeraad, Wilfred T V ; Wieten, Lotte ; Biessen, Erik A L ; Boon, Louis ; Neri, Dario ; Troost, Esther G C ; Dubois, Ludwig J ; Lambin, Philippe

Clinical cancer research : an official journal of the American Association for Cancer Research, 01 March 2015, Vol.21(5), pp.1151-60 [Peer Reviewed Journal]

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28
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Structural and functional conservation of key domains in InsP^sub 3^ and ryanodine receptors

Seo, Min-Duk ; Velamakanni, Saroj ; Ishiyama, Noboru ; Stathopulos, Peter ; Rossi, Ana ; Khan, Samir ; Dale, Philippa ; Li, Congmin ; Ames, James ; Ikura, Mitsuhiko ; Taylor, Colin

Nature, Mar 1, 2012, Vol.483(7387), pp.108-12 [Peer Reviewed Journal]

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29
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MAP65 coordinate microtubule growth during bundle formation

Stoppin-Mellet, Virginie ; Fache, Vincent ; Portran, Didier ; Martiel, Jean-Louis ; Vantard, Marylin

PloS one, 2013, Vol.8(2), pp.e56808 [Peer Reviewed Journal]

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30
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A potential peptide therapeutic derived from the juxtamembrane domain of the epidermal growth factor receptor

Boran, Aislyn D W ; Seco, Joseph ; Jayaraman, Vinodh ; Jayaraman, Gomathi ; Zhao, Shan ; Reddy, Sushmitha ; Chen, Yibang ; Iyengar, Ravi

PloS one, 2012, Vol.7(11), pp.e49702 [Peer Reviewed Journal]

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31
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Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy

Broderick, Ronan ; Ramadurai, Sivaramakrishnan ; Tóth, Katalin ; Togashi, Denisio M ; Ryder, Alan G ; Langowski, Jörg ; Nasheuer, Heinz Peter

PloS one, 2012, Vol.7(4), pp.e35537 [Peer Reviewed Journal]

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32
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Characterization of protective immune responses induced by pneumococcal surface protein A in fusion with pneumolysin derivatives

Goulart, Cibelly ; Da Silva, Thais Raquel ; Rodriguez, Dunia ; Politano, Walter Rodrigo ; Leite, Luciana C C ; Darrieux, Michelle

PloS one, 2013, Vol.8(3), pp.e59605 [Peer Reviewed Journal]

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33
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Linked production of pyroglutamate-modified proteins via self-cleavage of fusion tags with TEV protease and autonomous N-terminal cyclization with glutaminyl cyclase in vivo

Shih, Yan-Ping ; Chou, Chi-Chi ; Chen, Yi-Ling ; Huang, Kai-Fa ; Wang, Andrew H-J

PloS one, 2014, Vol.9(4), pp.e94812 [Peer Reviewed Journal]

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34
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High-throughput protein expression using a combination of ligation-independent cloning (LIC) and infrared fluorescent protein (IFP) detection

Dortay, Hakan ; Akula, Usha Madhuri ; Westphal, Christin ; Sittig, Marie ; Mueller-Roeber, Bernd

PloS one, 26 April 2011, Vol.6(4), pp.e18900 [Peer Reviewed Journal]

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35
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Structure analysis and conformational transitions of the cell penetrating peptide transportan 10 in the membrane-bound state

Fanghänel, Susanne ; Wadhwani, Parvesh ; Strandberg, Erik ; Verdurmen, Wouter P R ; Bürck, Jochen ; Ehni, Sebastian ; Mykhailiuk, Pavel K ; Afonin, Sergii ; Gerthsen, Dagmar ; Komarov, Igor V ; Brock, Roland ; Ulrich, Anne S

PloS one, 2014, Vol.9(6), pp.e99653 [Peer Reviewed Journal]

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36
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The Development of a Recombinant scFv Monoclonal Antibody Targeting Canine CD20 for Use in Comparative Medicine

Jain, Saurabh ; Aresu, Luca ; Comazzi, Stefano ; Shi, Jianguo ; Worrall, Erin ; Clayton, John ; Humphries, William ; Hemmington, Sandra ; Davis, Paul ; Murray, Euan ; Limeneh, Asmare A ; Ball, Kathryn ; Ruckova, Eva ; Muller, Petr ; Vojtesek, Borek ; Fahraeus, Robin ; Argyle, David ; Hupp, Ted R

PloS one, 2016, Vol.11(2), pp.e0148366 [Peer Reviewed Journal]

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37
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Actin fusion proteins alter the dynamics of mechanically induced cytoskeleton rearrangement

Deibler, Martin ; Spatz, Joachim P ; Kemkemer, Ralf

PloS one, 2011, Vol.6(8), pp.e22941 [Peer Reviewed Journal]

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