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High-Salt Diet Increases Sensitivity to NO and eNOS Expression But Not NO Production in THALs

Ortiz, A., Pablo ; Stoos, J., Barbara ; Hong, M., Nancy ; Boesch, F., Dianne ; Plato, L., Craig ; Garvin, L., Jeffrey

Hypertension: Journal of the American Heart Association, 2003, Vol.41(3, Part 2 Suppl), pp.682-687 [Peer Reviewed Journal]

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  • Title:
    High-Salt Diet Increases Sensitivity to NO and eNOS Expression But Not NO Production in THALs
  • Author: Ortiz, A., Pablo ; Stoos, J., Barbara ; Hong, M., Nancy ; Boesch, F., Dianne ; Plato, L., Craig ; Garvin, L., Jeffrey
  • Description: ABSTRACT—: l-Arginine inhibits thick ascending limb (THAL) NaCl absorption by activating endothelial NO synthase (eNOS) and increasing NO production. Inhibition of renal NO production combined with a high-salt diet produces hypertension, and the THAL has been implicated in salt-sensitive hypertension. We hypothesized that a high-salt diet enhances the inhibitory action of l-arginine on NaCl absorption by THALs because of increased eNOS expression and NO production. To test this, we used isolated THALs from rats on a normal-salt (NS) or high-salt diet (HS) for 7 to 10 days. l-Arginine (1 mmol/L) decreased chloride absorption by 56±10% in THALs from rats on a HS diet, but only 29±3% in THALs from rats on a NS diet. eNOS expression in isolated THALs from rats on a HS diet was increased by 3.9-fold compared with NS (P <0.03). However, l-arginine increased NO levels to the same extent in THALs from both groups, as measured with DAF-2 DA or a NO-sensitive electrode. To determine whether a HS diet increases the sensitivity of the THAL to NO, we tested the effects of the NO donor spermine NONOate on chloride absorption. In THALs from rats on a HS diet, 1 and 5 μmol/L spermine NONOate reduced chloride absorption by 35±5% and 58±6%, respectively. In contrast, these same concentrations of spermine NONOate reduced chloride absorption by 4±4% (P <0.03 versus HS diet) and 43±9% in THALs from rats on a NS diet. We conclude that a HS diet enhances the effect of NO in the THAL. l-Arginine–stimulated NO production was not enhanced by a HS diet, despite increased eNOS protein.
  • Is Part Of: Hypertension: Journal of the American Heart Association, 2003, Vol.41(3, Part 2 Suppl), pp.682-687
  • Identifier: ISSN: 0194-911X ; DOI: 10.1161/01.HYP.0000047872.07864.20
  • Source: Copyright © 2013 Lippincott Williams & Wilkins. All rights reserved.

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