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Induction of cyclooxygenase-2 in thick ascending limb cells by adrenalectomy

Vio, C P ; An, S J ; Céspedes, C ; Mcgiff, J C ; Ferreri, N R

Journal of the American Society of Nephrology : JASN, April 2001, Vol.12(4), pp.649-58 [Peer Reviewed Journal]

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  • Title:
    Induction of cyclooxygenase-2 in thick ascending limb cells by adrenalectomy
  • Author: Vio, C P ; An, S J ; Céspedes, C ; Mcgiff, J C ; Ferreri, N R
  • Description: Adrenalectomized (ADX) and sham-operated rats received either dexamethasone (DEX) or vehicle. Renal tissue was used for morphologic analysis, assessment of cyclooxygenase-2 (COX-2) protein expression and mRNA accumulation, and quantitation of COX-2 activity. In untreated or shamoperated rats, COX-2 protein was observed in a subset of tubular epithelial cells (30% of TAL cells expressed COX-2 in a manner consistent with recruitment of COX-2-positive TAL cells toward the medulla. Treatment of ADX rats with DEX reduced the number of COX-2-positive cells to that observed in sham-operated or intact rats. COX-2 mRNA accumulation was increased by ADX and partially attenuated by treatment with DEX. Western blot analysis of cortical microsomes revealed a substantial increase in COX-2 expression in ADX rats, compared with ADX/DEX-treated, sham-operated, or intact rats. The increase in COX-2 protein expression was associated with a twofold increase in prostaglandin E(2) formation by cortical microsomes obtained from ADX rats, compared with sham-operated rats. It is concluded that ADX induces expression of enzymatically active COX-2, such that expression occurs in the cortical TAL and proceeds in a defined pattern toward the outer medullary TAL. It is suggested that ADX induces expression of TAL cells that, in the basal state, do not express COX-2 protein.
  • Is Part Of: Journal of the American Society of Nephrology : JASN, April 2001, Vol.12(4), pp.649-58
  • Identifier: ISSN: 1046-6673 ; PMID: 11274225 Version:1
  • Subjects: Adrenalectomy ; Isoenzymes -- Metabolism ; Loop of Henle -- Enzymology ; Prostaglandin-Endoperoxide Synthases -- Metabolism
  • Language: English
  • Source: MEDLINE/PubMed (U.S. National Library of Medicine)

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