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Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells

Hennessy, Erica ; Clynes, Martin ; Jeppesen, Per Bendix ; O’driscoll, Lorraine

Biochemical and Biophysical Research Communications, 2010, Vol.396(2), pp.457-462 [Peer Reviewed Journal]

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  • Title:
    Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells
  • Author: Hennessy, Erica ; Clynes, Martin ; Jeppesen, Per Bendix ; O’driscoll, Lorraine
  • Description: MicroRNAs (miRNAs) are a family of endogenous small non-coding RNAs which regulate mRNAs at the post-transcriptional level. MiRNAs have been identified in both normal physiological and pathological conditions. To date, a limited number of miRNAs have been shown to be involved in the regulation of insulin secretion. We have identified a panel of 10 miRNAs down-regulated in glucose non-responsive MIN6 cells compared to glucose responsive cells using TaqMan Low Density miRNA Arrays. Of these 10 miRNA targets, subsequent functional investigations involving knockdown of mir-200a, mir-130a and mir-410 levels suggested that they may decrease the capability of MIN6 cells to secrete insulin in response to stimulatory levels of glucose. Conversely, experiment with over-expression of mir-410 suggest that it may enhance levels of glucose stimulated insulin secretion. In this study, we have also identified 21 miRNAs not previously known to have a potential murine homologue.
  • Is Part Of: Biochemical and Biophysical Research Communications, 2010, Vol.396(2), pp.457-462
  • Identifier: ISSN: 0006-291X ; E-ISSN: 1090-2104 ; DOI: 10.1016/j.bbrc.2010.04.116
  • Subjects: Microrna ; Gsis ; Insulin Secretion ; Min6 Cells ; Biology ; Chemistry ; Anatomy & Physiology
  • Language: English

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