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Studies on non-covalent associations of immunosuppressive drugs with serum albumin using pneumatically assisted electrospray ionization mass spectrometry

Bakhtiar, Ray ; Stearns, Ralph A.

Rapid Communications in Mass Spectrometry, 1995, Vol.9(3), pp.240-244 [Peer Reviewed Journal]

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  • Title:
    Studies on non-covalent associations of immunosuppressive drugs with serum albumin using pneumatically assisted electrospray ionization mass spectrometry
  • Author: Bakhtiar, Ray ; Stearns, Ralph A.
  • Description: Pneumatically assisted electrospray ionization (ion-spray) mass spectrometry was employed in the detection of non-covalent interactions of immunosuppressive drugs with proteins. Non-covalent complexes of albumin (rat and bovine) with FK506, dihydro-FK506, and FK520 were observed. No complexation was detected between albumin and cyclosporin A (CsA), even at a protein-ligand molar ratio of 1:20. It is suggested that the lack of detection of an albumin-CsA non-covalent complex may be attributed to dissociation of the weakly associated species during ion vaporization. Nonetheless, preliminary results indicate that ion-spray mass spectrometry has potential in qualitative evaluation of drug-plasma protein interaction.
  • Is Part Of: Rapid Communications in Mass Spectrometry, 1995, Vol.9(3), pp.240-244
  • Identifier: ISSN: 0951-4198 ; E-ISSN: 1097-0231 ; DOI: http://dx.doi.org/10.1002/rcm.1290090313
  • Subjects: Immunosuppressive Agents -- Blood
  • Language: English

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