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Fluorescent determination of chloride in nanoliter samples

García, Néstor H ; Plato, Craig F ; Garvin, Jeffrey L ; García, Néstor H

Kidney International, January 1999, Vol.55(1), pp.321-325 [Peer Reviewed Journal]

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  • Title:
    Fluorescent determination of chloride in nanoliter samples
  • Author: García, Néstor H ; Plato, Craig F ; Garvin, Jeffrey L ; García, Néstor H
  • Description: Fluorescent determination of chloride (Cl ) in nanoliter samples. Measurements of Cl in nanoliter samples, such as those collected during isolated, perfused tubule experiments, have been difficult, somewhat insensitive, and/or require custom-made equipment. We developed a technique using a fluorescent Cl indicator, 6-methoxy-N-(3-sulfopropyl) quinolinium (SPQ), to make these measurements simple and reliable. This is a simple procedure that relies on the selectivity of the dye and the fact that Cl quenches its fluorescence. To measure millimolar quantities of Cl in nanoliter samples, we prepared a solution of 0.25 mM SPQ and loaded it into the reservoir of a continuous-flow ultramicrofluorometer, which can be constructed from commercially available components. Samples were injected with a calibrated pipette via an injection port, and the resultant...
  • Is Part Of: Kidney International, January 1999, Vol.55(1), pp.321-325
  • Identifier: ISSN: 0085-2538 ; E-ISSN: 1523-1755 ; DOI: 10.1046/j.1523-1755.1999.00239.x
  • Subjects: Cotransport ; Furosemide ; Dids ; Bromide ; Kidney ; Thick Ascending Limb ; Medicine
  • Language: English
  • Source: ScienceDirect Journals (Elsevier)

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