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Characterization of rapid intervascular transport of cadmium in rice stem by radioisotope imaging

Kobayashi, Natsuko I ; Tanoi, Keitaro ; Hirose, Atsushi ; Nakanishi, Tomoko M

Journal Of Experimental Botany, 2013, Vol. 64(2), pp.507-517 [Peer Reviewed Journal]

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  • Title:
    Characterization of rapid intervascular transport of cadmium in rice stem by radioisotope imaging
  • Author: Kobayashi, Natsuko I ; Tanoi, Keitaro ; Hirose, Atsushi ; Nakanishi, Tomoko M
  • Description: Participation of the intervascular transport system within the rice stem during cadmium (Cd) partitioning was investigated by characterizing 109 Cd behaviour in the shoot. In addition, 45 Ca, 32 P, and 35 S partitioning patterns were analysed for comparison with that of 109 Cd. Each tracer was applied to the seedling roots for 15min, and the shoots were harvested either at 15min (i.e. immediately after tracer application) or at 48h. Distribution patterns of each element at 15min were studied to identify the primary transport pathway before remobilization was initiated. 32 P was preferentially transported to completely expanded leaf blades having the highest transpiration rate. The newest leaf received minimal amounts of 32 P. In contrast, the amount of 35 S transported to the newest leaf was similar to that transported to the other mature leaf blades. Preferential movement towards the newest leaf was evident for 109 Cd and 45 Ca. These results directly indicate that elemental transport is differentially regulated in the vegetative stem as early as 15min before the elements are transported to leaves. Cd behaviour in the stem was investigated in detail by obtaining serial section images from the bottom part of shoots after 109 Cd was applied to a single crown root. At 30min, the maximum amount of 109 Cd was distributed in the peripheral cylinder of the longitudinal vascular bundles (PV) and, interestingly, some amount of 109 Cd was transported downwards along the PV. This transport manner of 109 Cd provides evidence that Cd can be loaded on the phloem at the stem immediately after Cd is transported from the root.
  • Is Part Of: Journal Of Experimental Botany, 2013, Vol. 64(2), pp.507-517
  • Identifier: ISSN: 0022-0957 ; E-ISSN: 1460-2431 ; DOI: 10.1093/jxb/ers344
  • Subjects: Autoradiography ; Cadmium ; Calcium ; Phosphate ; Radioisotope ; Rice ; Sulphate ; Tracer ; Transport ; Xylem - To - Phloem

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