skip to main content
Language:
Search Limited to: Search Limited to: Resource type Show Results with: Show Results with: Index

A stable isotope dilution tandem mass spectrometry method of major kavalactones and its applications

Wang, Yi ; Eans, Shainnel O ; Stacy, Heather M ; Narayanapillai, Sreekanth C ; Sharma, Abhisheak ; Fujioka, Naomi ; Haddad, Linda ; Mclaughlin, Jay ; Avery, Bonnie A ; Xing, Chengguo

PloS one, 2018, Vol.13(5), pp.e0197940 [Peer Reviewed Journal]

Full text available

View all versions
Citations Cited by
  • Title:
    A stable isotope dilution tandem mass spectrometry method of major kavalactones and its applications
  • Author: Wang, Yi ; Eans, Shainnel O ; Stacy, Heather M ; Narayanapillai, Sreekanth C ; Sharma, Abhisheak ; Fujioka, Naomi ; Haddad, Linda ; Mclaughlin, Jay ; Avery, Bonnie A ; Xing, Chengguo
  • Description: Kava is regaining its popularity with detailed characterizations warranted. We developed an ultraperformance liquid chromatography high-resolution tandem mass spectrometry (UPLC-MS/MS) method for major kavalactones (kavain, dihydrokavain, methysticin, dihydromethysticin and desmethoxyyangonin) with excellent selectivity and specificity. The method has been validated for different matrices following the Food and Drug Administration guidance of analytical procedures and methods validation. The scope of this method has been demonstrated by quantifying these kavalactones in two kava products, characterizing their tissue distribution and pharmacokinetics in mice, and detecting their presence in human urines and plasmas upon kava intake. As expected, the abundances of these kavalactones differed significantly in kava products. All of them exhibited a large volume of distribution with extensive tissue affinity and adequate mean residence time (MRT) in mice. This method also successfully quantified these kavalactones in human body fluids upon kava consumption at the recommended human dose. This UPLC-MS/MS method therefore can be used to characterize kava products and its pharmacokinetics in animals and in humans.
  • Is Part Of: PloS one, 2018, Vol.13(5), pp.e0197940
  • Identifier: E-ISSN: 1932-6203 ; PMID: 29795658 Version:1 ; DOI: 10.1371/journal.pone.0197940
  • Subjects: Radioisotope Dilution Technique ; Kava -- Chemistry ; Lactones -- Administration & Dosage ; Tandem Mass Spectrometry -- Methods
  • Language: English

Searching Remote Databases, Please Wait