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Toxin profile, antibiotic resistance, and phenotypic and molecular characterization of Bacillus cereus in Sunsik

Chon, Jung-Whan ; Kim, Jong-Hyun ; Lee, Sun-Jin ; Hyeon, Ji-Yeon ; Seo, Kun-Ho

Food Microbiology, October 2012, Vol.32(1), pp.217-222 [Peer Reviewed Journal]

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  • Title:
    Toxin profile, antibiotic resistance, and phenotypic and molecular characterization of Bacillus cereus in Sunsik
  • Author: Chon, Jung-Whan ; Kim, Jong-Hyun ; Lee, Sun-Jin ; Hyeon, Ji-Yeon ; Seo, Kun-Ho
  • Description: , a ready-to-eat food in Korea, is comprised of various agricultural and marine products, and has been an important concern in food poisoning. The aim of this study was to investigate the toxin profiles, genotypic and phenotypic patterns as well as antibiotic resistance of strains isolated from . A subtyping method known as automated repetitive sequence-based PCR system (DiversiLab™) was used to assess the intraspecific biodiversity of these isolates. Thirty-five strains were isolated from 100 commercial samples, all of which harbored at least 1 enterotoxin gene. The detection rates of , and enterotoxin gene among all isolates were 97%, 86%, 77%, and 100%, respectively. Most strains also produced corresponding enterotoxins such as HBL (83%) and NHE (94%). One strain (2.9%) carried the emetic toxin genes, including and , and was positive for the HEp-2 cell emetic toxin assay. Most strains were positive for various biochemical tests such as salicin hydrolysis (86%), starch fermentation (89%), hemolysis (89%), motility test (100%) and lecithinase hydrolysis (89%). All isolates were susceptible to most antibiotics although they were highly resistant to β-lactam antibiotics. By using the automated rep-PCR system, all isolates were successfully differentiated, indicating the diversity of strains present in . ► We isolate strains isolated from ► We investigate toxin profiles, phenotype, antibiotic resistance, intraspecific biodiversity. ► All strains harbored at least 1 enterotoxin gene. ► Most strains were positive for enterotoxins and highly resistant to β-lactame antibiotics. ► need a stricter microbial standard and control for to ensure safety.
  • Is Part Of: Food Microbiology, October 2012, Vol.32(1), pp.217-222
  • Identifier: ISSN: 0740-0020 ; E-ISSN: 1095-9998 ; DOI: 10.1016/j.fm.2012.06.003
  • Subjects: Sunsik ; Bacillus Cereus ; Prevalence ; Characterization ; Biology ; Economics
  • Language: English

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