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An in vivo method for adenovirus-mediated transduction of thick ascending limbs

Ortiz, Pablo A ; Hong, Nancy J ; Plato, Craig F ; Varela, Marisela ; Garvin, Jeffrey L

Kidney International, March 2003, Vol.63(3), pp.1141-1149 [Peer Reviewed Journal]

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  • Title:
    An in vivo method for adenovirus-mediated transduction of thick ascending limbs
  • Author: Ortiz, Pablo A ; Hong, Nancy J ; Plato, Craig F ; Varela, Marisela ; Garvin, Jeffrey L
  • Description: An in vivo method for adenovirus-mediated transduction of thick ascending limbs. The thick ascending limb of the loop of Henle (THAL) plays an important role in the maintenance of salt, water, and acid-base balance. While techniques for gene transfer of renal vascular cells and some tubular segments have been described, in vivo transduction of THALs has not been successful. We hypothesized that in vivo injection of adenoviral vectors into the renal medulla would result in efficient transduction of THALs. We injected recombinant adenoviruses containing the reporter gene, green fluorescent protein (GFP), driven by either the cytomegalovirus promoter (Ad-CMVGFP) or the promoter for the Na/K/2 Cl cotransporter (Ad-NKCC2GFP), which is THAL-specific, into the outer medullary interstitium of Sprague-Dawley rat kidneys. Kidneys were removed at various times after viral injection and analyzed for GFP expression. Western blots revealed...
  • Is Part Of: Kidney International, March 2003, Vol.63(3), pp.1141-1149
  • Identifier: ISSN: 0085-2538 ; E-ISSN: 1523-1755 ; DOI: 10.1046/j.1523-1755.2003.00827.x
  • Subjects: Kidney Tubules ; Hypertension ; Nacl Transport ; Kidney Tubules ; Hypertension ; Nacl Transport ; Medicine
  • Language: English
  • Source: ScienceDirect Journals (Elsevier)

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