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DIETHYLENE GLYCOL DISTEARATE AS AN EMBEDDING MEDIUM FOR HIGH RESOLUTION LIGHT MICROSCOPY

Taleporos, Plato

Journal of Histochemistry and Cytochemistry, January 1974, Vol.22(1), pp.29-34 [Peer Reviewed Journal]

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  • Title:
    DIETHYLENE GLYCOL DISTEARATE AS AN EMBEDDING MEDIUM FOR HIGH RESOLUTION LIGHT MICROSCOPY
  • Author: Taleporos, Plato
  • Description: Owing to its physical properties, diethylene glycol distearate is especially suited for obtaining thin sections of high quality with little or no compression. Tissues can be cut as thin as 1 µm on a steel knife without cooling, but a water trough is essential. The wax is translucent, so that specimens can be oriented for a desired plane of sectioning. The wax is also of sufficient hardness to serve as an embedding medium for osmium-fixed material and can readily be removed from such tissue sections for rapid phase contrast studies. A general method is described for embedding and sectioning with diethylene glycol distearate wax and examples of freeze-substituted and osmium-fixed material are illustrated. It is concluded that this synthetic wax can offer a convenient and simple means for obtaining thin sections for most aspects of high resolution light microscopy.
  • Is Part Of: Journal of Histochemistry and Cytochemistry, January 1974, Vol.22(1), pp.29-34
  • Identifier: ISSN: 0022-1554 ; E-ISSN: 1551-5044 ; DOI: 10.1177/22.1.29
  • Subjects: Biology ; Anatomy & Physiology
  • Language: English

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