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Development of real-time PCR for the detection of Clostridium perfringens in meats and vegetables

Chon, Jung-Whan ; Park, Jong-Seok ; Hyeon, Ji-Yeon ; Park, Chankyu ; Song, Kwang-Young ; Hong, Kwang-Won ; Hwang, In-Gyun ; Kwak, Hyo-Sun ; Seo, Kun-Ho

Journal of microbiology and biotechnology, April 2012, Vol.22(4), pp.530-4 [Peer Reviewed Journal]

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Title:
Development of real-time PCR for the detection of Clostridium perfringens in meats and vegetables
Author: Chon, Jung-Whan ; Park, Jong-Seok ; Hyeon, Ji-Yeon ; Park, Chankyu ; Song, Kwang-Young ; Hong, Kwang-Won ; Hwang, In-Gyun ; Kwak, Hyo-Sun ; Seo, Kun-Ho
Description: A real-time PCR assay was developed and validated inhouse specifically for the detection of Clostridium perfringens (Cl. perfringens) in meats and vegetables by comparing with the culture method. The detection limit of the real-time PCR assay in phosphate-buffered saline was 10² CFU/ml. When the two methods were compared in food samples inoculated with Cl. perfringens, the culture method detected 52 positives, whereas real-time PCR detected 51 positives out of 160 samples. The difference was without statistical significance (p>0.05). Real-time PCR assay is an option for quality assurance laboratories to perform standard diagnostic tests, considering its detection ability and time-saving efficiency.
Is Part Of: Journal of microbiology and biotechnology, April 2012, Vol.22(4), pp.530-4
Identifier: E-ISSN: 1738-8872 ; PMID: 22534301 Version:1
Subjects: Clostridium Perfringens -- Isolation & Purification ; Food Contamination -- Analysis ; Meat -- Microbiology ; Real-Time Polymerase Chain Reaction -- Methods ; Vegetables -- Microbiology
Language: English

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