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Butyrate stimulates the secretion of apolipoprotein B-100-containing lipoproteins from HepG2 cells by inhibiting the intracellular degradation

Kaptein, Allard ; de Wit, Elly C.M ; Princen, Hans M.G

Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, 1994, Vol.1213(3), pp.349-356

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  • Title:
    Butyrate stimulates the secretion of apolipoprotein B-100-containing lipoproteins from HepG2 cells by inhibiting the intracellular degradation
  • Author: Kaptein, Allard ; de Wit, Elly C.M ; Princen, Hans M.G
  • Description: We have shown previously that sodium butyrate induces a 2-fold increase in the secretion of apo B-100 by HepG2 cells. The apo B-100 mRNA level was not changed in butyrate-treated cells, indicating regulation at the translational or co- or posttranslational level (Biochem. J. (1991) 278, 557–564). In this paper, the mechanism by which butyrate increases apo B-100 secretion was further investigated. Pulse-chase analysis showed that in control incubations only 18 ± 4% of the total amount of labelled apo B-100, present intracellularly after a 10 min pulse period, was secreted after a 90 min chase period, indicating that the major part of newly synthesized apo B-100 is degraded intracellularly. After addition of butyrate the secreted amount increased to 32 ± 6% of the total synthesized amount. Treatment of HepG2 cells with butyrate resulted in an enhanced intracellular concentration of triacylglycerols (+ 30%), with no or only a marginal effect on the cellular content of cholesterol...
  • Is Part Of: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, 1994, Vol.1213(3), pp.349-356
  • Identifier: ISSN: 0005-2760 ; DOI: 10.1016/0005-2760(94)00064-6
  • Subjects: Apo B-100 ; Apo A-I ; Posttranslational Regulation ; Intracellular Degradation ; Butyrate ; Hepg2 ; Apo B-100 ; Apo A-I ; Posttranslational Regulation ; Intracellular Degradation ; Butyrate ; Hepg2 ; Fbs ; Lpds ; Apo ; Chemistry
  • Language: English
  • Source: ScienceDirect Journals (Elsevier)

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