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Acute intravenous glucose load impairs early insulin secretion and insulin content in islet β cells in mice

Yan, Dien ; Zhao, Yinan ; Gao, Xiuying ; Zhou, Yingsheng

Life Sciences, 01 January 2016, Vol.144, pp.148-155 [Peer Reviewed Journal]

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  • Title:
    Acute intravenous glucose load impairs early insulin secretion and insulin content in islet β cells in mice
  • Author: Yan, Dien ; Zhao, Yinan ; Gao, Xiuying ; Zhou, Yingsheng
  • Description: To investigate insulin secretion and content in islet β cells after intravenous glucose load in mice. Acute hyperglycemia (≥ 16.7 mmol/L) in C57BL/J6 mice was achieved by hyperglycemic clamp. Mice were divided into four groups: a 2-hour and a 4-hour high glucose-infusion (2 h-HG and 4 h-HG) with 25% dextrose groups and control groups with saline infusion of the same duration. Insulin levels and response were measured using intraperitoneal glucose tolerance test (IPGTT) in mice and glucose-stimulated insulin secretion (GSIS) for isolated islets after overnight culture. Immunohistochemistry and electron microscopy (EM) for islet β cells were used after the hyperglycemic clamp to study morphologic changes of insulin granules and to assess the impact of acute glucose load on islet histology. Blood glucose at 15, 30, 60 and 120 min was significantly higher...
  • Is Part Of: Life Sciences, 01 January 2016, Vol.144, pp.148-155
  • Identifier: ISSN: 0024-3205 ; E-ISSN: 1879-0631 ; DOI: 10.1016/j.lfs.2015.12.012
  • Subjects: Acute Hyperglycemia ; Ipgtt ; Gsis ; Insulin Granules ; Sciences (General) ; Biology
  • Language: English

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