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Lifelong reporter gene imaging in the lungs of mice following polyethyleneimine-mediated sleeping-beauty transposon delivery

Lin, Erh-Hsuan ; Keramidas, Michelle ; Rome, Claire ; Chiu, Wen-Ta ; Wu, Cheng-Wen ; Coll, Jean-Luc ; Deng, Win-Ping

Biomaterials, 2011, Vol.32(7), pp.1978-1985 [Peer Reviewed Journal]

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  • Title:
    Lifelong reporter gene imaging in the lungs of mice following polyethyleneimine-mediated sleeping-beauty transposon delivery
  • Author: Lin, Erh-Hsuan ; Keramidas, Michelle ; Rome, Claire ; Chiu, Wen-Ta ; Wu, Cheng-Wen ; Coll, Jean-Luc ; Deng, Win-Ping
  • Description: Polyethyleneimine (PEI) is a cationic polymer that is effective in gene delivery in vivo. Plasmid DNA incorporating the Sleeping-Beauty (SB) transposon has been shown to induce long-term transgene expression in mouse lungs after PEI-mediated delivery. In the current report, we followed the reporter gene expression mediated by PEI/SB delivery in lungs of mice using the non-invasive bioluminescent imaging (BLI) technology. After delivery, the reporter gene signal showed a rapid decay in the first two weeks to a nearly undetectable level, but then the signal augmented gradually in the following weeks and finally reached a stable level that maintained until the natural death of animals. The stabilization of transgene expression is associated with the multiplication of a small number of PEI/SB-labeled alveolar cells, which proliferated both under normal conditions and in response to acute local injury for epithelia repair, and may play a role in long-term homeostatic maintenance in...
  • Is Part Of: Biomaterials, 2011, Vol.32(7), pp.1978-1985
  • Identifier: ISSN: 0142-9612 ; E-ISSN: 1878-5905 ; DOI: 10.1016/j.biomaterials.2010.11.026
  • Subjects: Non-Invasive Bioluminescent Imaging (Bli) ; Polyethyleneimine (PEI) ; Sleeping-Beauty (SB) ; Alveolar Epithelial Cells (Aecs) ; Medicine ; Engineering
  • Language: English
  • Source: ScienceDirect Journals (Elsevier)

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